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Technical Brief: Siliconizing Glassware
1. Siliconizing Glassware
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| | | For siliconizing glassware, we recommend the use of Sigmacote®, catalog no. SL-2, available from Sigma Chemical Co., P.O. Box 14508, St. Louis, MO 63178. (tel: 800-325-5832) According to the manufacturer's instructions, the product is ready to use without dilution and the following procedure is provided by Sigma:
| | | 1.1 | Begin the procedure by cleaning all glassware to be used.
| | | 1.2 | Apply the Sigmacote® product, thoroughly coating the surface of all the clean glassware to be exposed to microcarriers. (For example, the inside of the glass vessel.)
| | | 1.3 | Drain excess Sigmacote® from the glassware and allow to air-dry. (18-24 hours typically)
| | | 1.4 | Wash and rinse the glassware again before a final thorough rinsing with DI water. |
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Use of Recombinant and Synthetic Peptides 